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Fig. 3. TAJ-induced non-apoptotic cell death is independent of caspase activation. (A) DEVDase activity assay. 293T cells were transfected with the empty vector or indicated constructs and, 40 hours after transfection, experiments were performed as described in Materials and Methods. All data are presented as mean±standard deviation (s.d.). (B) Cellular proteins were extracted from 293T cells that were transfected with mock, Bax or TAJ/TROY plasmid. Protein levels were detected by western blots with various antibodies as described in Materials and Methods.
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