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Fig. 4. Mitochondrial changes in TAJ/TROY-transfected cells. (A) Transfected cells were subjected to mitochondrial transmembrane potential assessment 30 hours after transfection using a potentiometric dye, rhodamine 123. Loss of 
m was visualized as a reduction in the fluorescence signal in the FL1 channel. The proportions of cells with reduced rhodamine 123 staining are shown. Data are presented from one experiment. Experiments were performed in triplicate with similar results. (B) Ultrastructural mitochondrial characteristics of TAJ/TROY-induced cell death. HEK293, HeLa and 293T cells were transfected with a vector control or plasmids encoding Bax or TAJ/TROY along with a GFP-encoding vector. Extensive mitochondria swelling was seen in TAJ/TROY-transfected HEK293 (b,c), HeLa (e) and 293T (g-i) cells. The following micrographs of empty-vector-transfected HEK293 (a), HeLa (d) and 293T (f) cells provide normal cell controls. Scale bars, 1 µM (a,b,d,e), 0.5 µM (c,f-h), 0.2 µM (i).
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