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Fig. 6. Upregulation of PDCD5 protein in TAJ/TROY-transfected cells. (A) 293T cells were transfected with empty plasmid vector or indicated plasmids. 30 hours after transfection, endogenous PDCD5 levels were measured with a FITC-labeled anti-PDCD5 monoclonal antibody by flow cytometry. Median values of fluorescence intensity represent the expression of cellular PDCD5. 293T cells transfected with PDCD5 expression plasmid were used as a positive control. Significant upregulation of endogenous PDCD5 was detected in TAJ/TROY-overexpressing cells. (B) Schematic overlap of fluorescence histograms. It helped to observe the increase of median values of fluorescence intensity in TAJ/TROY-transfected cells.





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