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Fig. 1. Specificity of the anti-CRM1 and anti-Ran monoclonal antibodies. (A) Western blot of extracts from C. tentans tissue culture cells and salivary gland cells, probed with the anti-CRM1 antibody. (B) Western blot analysis of the expression of the N-terminal part of Ct-CRM1 (residues 1-329) in E. coli. Lane 1, before isopropyl ß-D-thiogalactopyranoside (IPTG) induction of expression. In lane 2, a polypeptide migrating at approximately 36 kDa (arrow) was recorded after induction by IPTG. (C) Western blot of protein extracts from C. tentans tissue culture cells (lane 1) and salivary gland cells (lane 2), probed with the anti-Ran antibody. (D) Expression of a GST-Ct-Ran fusion protein in E. coli. In lane 1, no protein was seen before induction with IPTG. In lane 2, the fusion protein (arrow) was detected as well as smaller polypeptides, specifically reacting with the anti-Ran antibody. In A-D, M indicates the positions of size markers (in kDa).





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