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Fig. 2. Localisation of Ct-CRM1 and Ct-Ran in C. tentans cells. (A-C) Localisation of Ct-CRM1. Diploid tissue culture cells (A), and salivary gland cell (B). NE, nuclear envelope. The cells were immunostained with the anti-CRM1 antibody and a FITC-conjugated secondary antibody. (C) Immunoelectron microscope image of part of the nuclear membrane of a salivary gland cell. Gold particles (arrows) show the location of Ct-CRM1. Bar, 400 nm. (D-E) Localisation of Ct-Ran. (D) Tissue culture cells, showing mainly staining of the nuclei. (E) Salivary gland cells. Bars in A and C, 10 µm; bars in B and E, 20 µm.