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Fig. 4. Immunoelectron microscopy localisation of Ct-CRM1 in salivary gland cell nuclei. (A) View of a region of an active BR gene locus. Short stretches of many different transcribing BR genes and of different parts of the BR gene are seen. Nascent BR pre-mRNP particles are labelled by the secondary, gold-conjugated antibody. The gold particles are approximately 6 nm in diameter. (B) A complete active BR gene is shown schematically with the growing BR pre-mRNP particles. At the proximal (p) part of the gene, the BR pre-mRNPs form short fibres. These fold into larger and larger spherical pre-mRNP particles seen in the middle (m) and distal (d) parts of the active gene. (C-F) sections highlight areas containing essentially proximal, middle and distal parts of the gene, respectively (the sizes of the nascent mRNPs are different according to the drawing in B.) Gold particles can be seen labelling the BR pre-mRNP in all three cases (arrows). Bars, 200 nm.





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