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Fig. 5. Ct-Ran is bound to chromatin, but also to nascent BR pre-mRNP. Anti-Ran staining of isolated chromosome IV (A) and chromosome I (B); Bar, 10 µm. In chromosome IV, the decondensed BR gene loci are only weakly stained (arrows). (C) Chromosome IV and III staining with the anti-Ran antibody is similar to staining of the chromosomal DNA with DAPI. In addition, the nucleolus on chromosome III was labelled. The two chromosomes are also shown in phase contrast. (D) RNase treatment before staining with the anti-Ran antibody did not significantly influence the staining pattern (except in the nucleolus). Bar, 10 µm. (E) Immunoelectron microscope analysis of Ct-Ran in a section through a BR gene. Gold labelling is seen of compact chromatin (Ch, thin arrows), of interchromatin regions between segments of transcribing BR genes (thick arrows), and of nascent BR pre-mRNP particles (arrowheads). Bar, 300 nm.





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