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Fig. 6. Immunoprecipitation of hnRNP complexes. Western blot of C. tentans nuclear proteins immunoprecipitated with the anti-hrp23 antibody. The blot was probed with a mixture of antibodies directed against hrp36 (similar to the mammalian hnRNP A/B proteins) (Visa et al., 1996), hrp23, a homologue of the Drosophila RSF1 protein (Labourier et al., 1999), CRM1 and Ran. Ct-CRM1, Ct-Ran, hrp36 and hrp23 were specifically detected (lane 3). A nuclear extract incubated with Protein A-Sepharose beads coupled to anti-mouse antibodies and anti-von Willebrand antibodies did not result in any precipitation of the proteins (lane 2). 1/80 of the nuclear extract used for immunoprecipitation is shown in lane 1. The bands migrating at approximately 55 kDa and 24 kDa were present in the negative control. In lane 3, the 24 kDa band is partly hidden by the Ct-Ran band.
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