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Fig. 3. The effect of inhibitors of various signal transduction agents on PAF-induced Ca2+ transients in two-cell embryos. Traces show the mean intracellular calcium of at least 30 embryos from three replicates. Embryos were treated with PAF:acetylhydrolase, then with inhibitors shown for 30 minutes and then challenged with 37 nM PAF/ml in the presence of inhibitor.