QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 4. (A) The effect of inhibitors of phosphoinositide 3-kinase on PAF-induced Ca²⁺ transients in two-cell embryos. The results are the mean and s.e.m. of at least 30 embryos from three replicates. Embryos were treated with PAF:acetylhydrolase, then with the inhibitors and concentrations shown and then challenged with 37 nM PAF/ml in the presence of inhibitors. (B) The expression of phosphoinositide 3-kinase subunits in the two-cell embryo. A: mRNA for P110 catalytic subunits in two-cell embryos and brain tissue. M is molecular weight size markers (GeneRuler DNA Ladder Mix). Lane 1 lacked reverse transcriptase; Lane 2, no template acted as a negative controls; Lane 3, ß-actin (243 bp; positive control); Lane 4, p110 ${alpha}$ (238 bp); Lane 5, p 110ß (146 bp); Lane 6, p110g (111 bp); and Lane 7, p110 ${delta}$ (bp 294). B: mRNA for P85 regulatory subunits in two-cell embryos. M is molecular weight size markers. Lane 1, no reverse transcriptase; Lane 2, no template; Lane 3, two-cell ß-actin; Lane 4, P85 ${alpha}$ -subunit (128bp), Lane 8 P85, ß-subunit (159 bp).

Return to article