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Fig. 6. The effect of the phosphoinositide 3-kinase inhibitor LY294002 on the development of zygotes in vitro. Zygotes were cultured individually in 10 µl drops of media for 96 hours. The three groups of bars in A and B show the results for incubation with 0 µl, 3 µl and 15 µl LY294002, respectively. (A) The proportion of embryos achieving expected developmental landmarks at each 24 hour interval was assessed, and (B) for those zygotes that developed to morphologically normal blastocysts after 96 hours culture the number of cells per embryo (mean ± s.d.) with apparently normal or obviously fragmented nuclei was assessed.
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