spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Mad2 is recruited to kinetochores after Bub1, BubR1 and Cenp-E. (A) A time line for the association of checkpoint proteins to the kinetochore during mitosis. (B) Protein extracts from DLD-1 cells expressing Myc or Myc-Mad2 were analysed by western blotting with anti-Mad2 antibody (SM2.2) or anti-Myc antibody (9E10). (C,D) Immunofluorescence images of DLD-1 Myc-Mad2 cells in different stages of mitosis stained to detect Myc-tagged Mad2 (9E10) and either Bub1 (SB1.3) (C) or BubR1 (SBR1.1). Cells were also stained to detect Aurora A (RAA.1) in order to define the spindle axis. (E) Quantitation of Cenp-E and Mad2 levels at kinetochores, normalized to the ACA signal, showing that Myc-Mad2 accumulates at kinetochores in late prometaphase, after Cenp-E.





Right arrow Return to article