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Fig. 2. Design of siRNA duplexes and repression of Bub1, Cenp-F and Survivin. (A) siRNA duplexes used in this study showing target sequence (AA-N19) including a scrambled siRNA duplex control. The control siRNA duplex was designed by scrambling the Bub1 target sequence and checking specificity in a BLAST search. (B) Immunoblots of asynchronous HeLa cell lysates 48 hours after transfection showing repression of Bub1, Cenp-F and Survivin. Tubulin was used as a loading control.





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