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Fig. 4. BubR1 is required for kinetochore localization of Cenp-E. DLD-1 or DLD-1 Myc-Mad2 cells were transfected with control or BubR1 siRNA duplexes, treated with nocodazole and then fixed and stained to detect BubR1 (blue), centromeres/kinetochores (ACA, green), DNA and Bub1, Myc-Mad2, Cenp-E or Cenp-F (red). (A) Representative projected image stacks showing that repression of BubR1 reduces kinetochore localization of Cenp-E but does not affect Bub1, Cenp-F or Mad2. Scale bar, 5 µm. (B) Normalized pixel intensities in control (black bar) and BubR1-repressed (white bar) cells. Values represent the mean and s.e.m. of at least 30 kinetochores in three different cells.





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