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Fig. 5. Cenp-E is required for efficient localization of Mad2 and Cenp-F. DLD-1 or DLD-1 Myc-Mad2 cells were transfected with control or Cenp-E siRNA duplexes, treated with nocodazole and then fixed and stained to detect Cenp-E (blue), centromeres/kinetochores (ACA, green), DNA and Bub1, BubR1, Myc-Mad2 or Cenp-F (red). (A) Representative projected image stacks showing that repression of Cenp-E reduces kinetochore localization of Mad2 and Cenp-F but does not affect Bub1 or BubR1. Scale bar, 5 µm. (B) Normalized pixel intensities in control (black bar) and Cenp-E-repressed (white bar) cells. Values represent the mean and s.e.m. of at least 30 kinetochores in three different cells.





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