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Fig. 1. p130Cas transiently associates with the estrogen receptor {alpha}, c-Src and the p85 subunit of PI 3-kinase (PI3K). T47D cells grown to confluence were made quiescent for rapid estrogen signaling by incubating them in phenol red-free RPMI-1640 medium supplemented with charcoal-treated serum for 24 hours. Cells were then left untreated or treated with 10 nM E2 for the indicated times (in hours) and detergent extracted. Cell lysates were immunoprecipitated with antibody to estrogen receptor {alpha}. Material co-immunoprecipitated with estrogen receptor were run on an 8% SDS-PAGE gel and immunoblotted with antibodies to p130Cas, c-Src, estrogen receptor {alpha} and p85 subunit of PI 3-kinase. The data reported here are a representative experiment out of five independent experiments.





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