spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Effect of FGF-2 and NGF on the differentiation of PC12 cells with respect to morphology and levels of MAP1B and tau. (A) Representative cell morphologies during differentiation (4 and 10 days) of PC12 cells in the presence of NGF or FGF-2. Note that PC12 cells after differentiation for 10 days with NGF have a larger number of thicker neurites than those with FGF-2. Bar, 50 µm. (B) Western blot showing MAP1B in PC12 cells differentiated with NGF or FGF-2 from 0 to 10 days. (C) Quantitative analysis of total tau and MAP1B levels in PC12 cells differentiated with NGF or FGF-2. Immunoreactivities (IR) of tau or MAP1B were converted based on the value (PSL) of these proteins from undifferentiated cells as 100%. Tau was analyzed on 125I western blots with Tau-1 after 8 hours dephosphorylation on the membrane with alkaline phosphatase. The increase of tau expression after 7 days of differentiation was confirmed with the phospho-independent tau antibody R134d and was 3.5 and 4 times higher (data not shown). MAP1B was analyzed with mAb 3G5.





Right arrow Return to article