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Fig. 3. Secretion of the C-terminal domain of Hsp150 in a sec24-1 mutant. sec24-1 (H1500, A), sec13-1 (H1429, B) and control cells (H1508, C) were preincubated for 15 minutes, 35S-labeled for 5 minutes and chased as indicated, at 37°C. Cell lysate samples (c) and the respective medium samples (m) were subjected to immunoprecipitation with Hsp150 antiserum and SDS-PAGE (15% gels) analysis. SUI-Cterm and released Cterm are indicated.





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