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Fig. 5. Electron microscopy of Hsp150{Delta}-HRP. sec24-1 (A; H1458), sec23-1 (C; H1459) and sec7-1 (D, H1455) mutants expressing Hsp150{Delta}-HRP, and the parental sec7-1 strain (B; H3) were incubated for 1 hour at 37°C, fixed and processed for HRP EM. Large black arrows indicate putative stained ER (A and C), or Golgi cisternae (D). The large open arrow points to unidentified unstained membrane (A). Small arrows indicate stained (C) and unstained (D) nuclear membrane. CW, cell wall; G, Golgi; N, nucleus; PM, plasma membrane; V, vacuole. Bars, 0.5 µm.





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