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Fig. 6. The C-terminal domain of Hsp150 as mediator of ER exit of invertase. (A) Control (H1540 in lane 1, and H1577 in lane 4), sec24-1 (H1544 in lane 2, and H1579 in lane 5), sec18-1 (H1542 in lane 3, and H1575 in lane 6), and sec13-1 (H1578 in lane 7) cells were 35S-labeled for 5 minutes after a 15-minute preincubation, and chased with CHX for 40 minutes at 37°C. After immunoprecipitation with Hsp150 antiserum and endo H digestion, the samples were subjected to SDS-PAGE analysis. The recombinant strains expressed SUI-Cterm-invertase or SUI-invertase-Cterm, as indicated. The apparent molecular weights of the immunoprecipitates (kDa) are indicated on the right. (B) sec18-1 (H1542), control (H1540), sec24-1 (H1544) and sec13-1 (H1578) cells were labeled as above, but immunoprecipitated with CPY antiserum. The ER (p1, 67 kDa), mature (m, 62 kDa) and cytosolic (c, 59 kDa) forms are indicated.





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