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Fig. 2. Immunolocalization of cell junctional proteins. Proteins were detected with the indicated antibodies. Endogenous and ectopically expressed E-cadherin was detected using an anti-E-cadherin antibody. Only ectopically expressed E-cadherin was detected with an anti-myc antibody. Whereas no cell contact staining pattern of tight junctional proteins could be observed in MDCK-Sna cells, the additional ectopic expression of E-cadherin restored a minimal cell contact staining pattern.





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