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Fig. 6. RT-PCR/southern bolt analysis revealed changes in the relative p120 isoform levels in Snail-induced epithelial to mesenchymal transitions. (A) Schematic representation of four possible start sites, resulting from N-terminal splicing variations. The alternatively spliced 407 bp sequence is deleted from the N-terminus in isoform 3 cDNA. The primer positions for RT-PCR and the regions used to synthesize southern blot probes (probe 1, 2) are also depicted. (B) Using probe 1, N-terminally spliced shorter fragments were detected in MDCK-neo cells, while longer fragments derived from the fibroblastic isoform cDNA (isoform 1) were detected in MDCK-Sna and MDCK-Sna-Ecad cells by RT-PCR/Southern blot analysis. Only the longer fragments were detected using a splice region-specific probe (probe 2).





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