spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Induction of NOS2 proceeds with the concomitant downregulation of the cav-1, cav-2 and cav-3 levels in mouse C2C12 myotubes treated with LPS/IFN-{gamma}. Myotubes were treated with LPS/IFN-{gamma} and the changes in NOS2, cav-1, cav-2 and cav-3 were analysed by immunoblot and quantified using the UVI-band software. Induction of NOS2 together with the accumulation of nitrites is depicted in (A) The intensity of the NOS2 bands at five different times as integrated using the UVI-band software is shown at the top. Accumulated nitrites in a 54 hour period is shown at the bottom. The disappearance of cav-1 (continuous line), cav-2 (dotted line) and cav-3 (dashed line) in myotubes is represented in (B), both as the immunodetected bands (top) and as a plot (bottom). Protein levels are expressed as percentage of the maximum; the initial amount of caveolin is therefore 100%. ß-Tubulin is also shown as a control of total protein in each sample. The results shown are representative of four experiments (means±s.d.).





Right arrow Return to article