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Fig. 3. Localization of NOS2-GFP to the Golgi apparatus (A) and particulate immunofluorescence of NOS2-GFP both in myoblasts and myotubes (B). Live C2C12 myoblasts were grown on glass coverslips, transfected with a NOS2-GFP construct and incubated with the Golgi marker BODIPY-Texas red ceramide (A). In addition, C2C12 myoblasts and myotubes were grown on glass coverslips and transfected with GFP alone (left) or with a construct of NOS2 fused to the GFP reporter (right) (B). (B, far right) Magnification of myotubes transfected with a NOS2-GFP construct. The subcellular localization was analysed by laser confocal microscopy at an excitation wavelength of 488 nm. Scale bar, 10 µM (bottom), 50 µM (all others). In all cases, the position of the cell nuclei (blue) was obtained after staining with Hoechst and excitation at 405 nm.
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