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Fig. 4. Keratin expression on CDD-induced pancreatitis. (A) Mice were fed a CDD for 3 days, followed by harvesting of pancreata from CDD-fed or from normal-diet-fed mice at various time-points then fixing and staining with hematoxylin and eosin. a=control diet; b=3 days of CDD feeding; c=3 days of CDD feeding then 1 day or normal diet; d=3 days of CDD feeding then 7 days or normal diet. (B) Pancreata were isolated from control-diet-fed (C) or CDD-fed mice. Feeding was done for 3 days followed by switching to a normal diet for 1 day (4d), 2 days (5d), 5 days (8d) or 7 days (10d). Pancreas fragments were solubilized in sample buffer, then equal amounts of protein (20 µg/lane) were analyzed (two separate mice/time-point) by blotting with antibodies to the indicated proteins.
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