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Fig. 5. Keratin expression after hyperthermia and water-immersion stresses. (A) Mouse core body temperature was increased to 42°C and maintained at this temperature for 20 minutes. Mice were then returned to their normal environment and allowed to maintain their usual activity and food/water intake. Pancreata were harvested from control non-heat exposed mice (C) or from the heat-stressed mice 0, 3, 6, 24 and 48 hours after the heat exposure. Two mice were used for each condition. Pancreatic total tissue homogenates were prepared and immunoblotted with antibodies to the indicated antigens. (B) Mice were immersed in a water bath (23°C) for 6 hours as described in Materials and Methods, then returned to their cages and allowed to maintain their usual activity and food/water intake. Pancreata were harvested from control non-water-immersed mice (C) or from the water-immersed mice 3, 6, 9 and 12 hours after removal from the water immersion. Total pancreatic protein extracts were prepared then analyzed by blotting as in A. Two mice were used for each condition.
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