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Fig. 1. Characterization of anti-{alpha}B-crystallin C-terminal (C1) and N-terminal (N1) peptide antibodies. (A) Coomassie-blue-stained gels of rat soleus muscle homogenate (lane 1), total L6E9 cell lysate (lane 2), purified {alpha}B-crystallin (lane 3) and tubulin (lane 4), and immunoblots with C1 antibody (lanes 5-8; corresponding to lanes 1-4) and N1 antibody (lanes 9-12; corresponding to lanes 1-4). (B) C1 antibody recognizes {alpha}B-crystallin in L6E9 cells (bottom right) as confirmed by a loss of reaction it after preadsorption of the antibody with the C-terminal peptide (bottom left). Scale bar, 25 µm.





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