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Fig. 4. Binding of ${alpha}$ B-crystallin to reconstituted MTs. (A) CBB-stained gel of co-precipitated ${alpha}$ B-crystallin and MAP-MTs. All lanes contain 1 mg ml^–1 MAP-MTs. The other lanes also contain: 2.5 µM ${alpha}$ B-crystallin (lane 2); 5 µM ${alpha}$ B-crystallin (lane 3); 10 µM ${alpha}$ B-crystallin (lane 4); 20 µM ${alpha}$ B-crystallin (lane 5); 40 µM ${alpha}$ B-crystallin (lane 6); 80 µM ${alpha}$ B-crystallin (lane 7). Arrowhead indicates ${alpha}$ B-crystallin band. (B) CBB-stained gel of co-precipitated ${alpha}$ B-crystallin and PCT-MT. All lanes contain 10 µM PCT-MT. The other lanes also contain: 5 µM ${alpha}$ B-crystallin (lane 2); 10 µM ${alpha}$ B-crystallin (lane 3); 20 µM ${alpha}$ B-crystallin (lane 4); 40 µM ${alpha}$ B-crystallin (lane 5) and 80 µM ${alpha}$ B-crystallin (lane 6). (C) The amount of co-precipitated ${alpha}$ B-crystallin was plotted for input ${alpha}$ B-crystallin. Closed circle represented for MTP-MTs and open circle for PCT-MTs.

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