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Fig. 6. Qualitative analysis of FGF-2 export in the absence and presence of aminopterin. Doxicycline-induced CHOFGF-2-GFP-DHFR cells were grown on coverslips in the absence (A,B) or presence (C-F) of aminopterin. Cells were fixed without permeabilization using paraformaldehyde followed by immunostaining using either affinity-purified anti-GFP antibodies (B,D) or monoclonal anti-His-tag antibodies (F). Total GFP fluorescence (A,C,E) as well as cell-surface staining (B,D,F) were analysed using a Zeiss LSM 510 confocal microscope.





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