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Fig. 4. Microtubule-dependent localization of IIGP to the Golgi. (A) subfraction of IIGP (A,C) localizes to the Golgi in IFN-stimulated murine BMM revealed by co-localization with the Golgi-resident protein -mannosidase (B,C). Imaging specifically the juxtanuclear accumulation of mHk3 (E,F) reveals co-localization with the cis-Golgi matrix protein GM130 (D,F) as well as nearby accumulation which is reminiscent to the position of the MTOC (data not shown). The IFN-stimulated BMM were treated for 1 hour with nocodazole to disrupt microtubules (G-L). The fine reticulate localization of IIGP in untreated cells (A and Fig. 3), appears contracted upon nocodazole treatment, most probably reflecting the loss of the microtubule-dependent extension of the ER into the cellular periphery (G,I,J,L) (Terasaki et al., 1986). Dispersion of the -mannosidase staining reflects the formation of Golgi-like structures at transitional ER exit sites (H,I) and colocalization with IIGP is not evident (G,I). Likewise, nocodazole treatment induces a scattered distribution of mHk3 staining (K,L).

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