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Fig. 7. Dephosphorylation of kinetochore phosphoepitopes releases BubR1 and Bub3. S2 cells were lysed with detergent either in the presence (T+Mc) or in the absence (T-Mc) of microcystin, fixed and immunostained. (A) Immunostaining with anti-BubR1 (green), 3F3/2 (red) and anti-CID (pink) antibodies shows that BubR1 staining is no longer detectable after dephosphorylation. CID localization is shown as a control and is not affected by dephosphorylation. (B) Immunostaining with anti-Bub3, 3F3/2 and anti-CID antibodies shows that Bub3 staining is lost after dephosphorylation, while CID labelling remains. Scale bar: 5 µm.





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