spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. GFP-ERK5 accumulates in the nucleus without anchoring upon LMB treatment. (A) HeLa cells were grown on coverslips and transfected with GFP-ERK5 construct (2.5 µg DNA) using the PEI method. The cells were either treated with LMB (5 ng/ml, 1 hour) or left untreated (NT), and then were washed with PBS, fixed with 3% PFA, permeabilized with 0.2% Triton X-100 and stained with DAPI. (B) HeLa cells were grown and treated as in A. After LMB treatment, cells were subjected to an in situ detergent extraction with NP-40 (0.2%, 5 minutes), stained with DAPI and examined with a fluorescence microscope.





Right arrow Return to article