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Fig. 6. MEK5 is localized in the nucleus. (A) HeLa cells were grown as described above, serum-starved, and then either stimulated with EGF (50 ng/ml, 15 minutes) or left unstimulated (NS). Then the cells were washed, fixed and stained with anti-MEK5 antibody and DAPI. (B) Rat-1 cells were grown and stimulated as described above, and then stained with anti-MEK5 antibody and DAPI. A blocking peptide (50 µg/ml) was added to the staining (lower panel) for competition. (C) Specificity of the anti-MEK5 antibody was demonstrated by western blot with extracts (30 µg) from HeLa and Rat-1 cells.





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