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Fig. 8. LMB does not affect ERK5 activation but does affect its down regulation. HeLa cells were grown in 6 cm plates, serum-starved and preincubated with LMB (5 ng/ml, 1 hour) or left untreated (NT), followed by EGF stimulation (50 ng/ml) for the indicated times. Then, the cells were harvested in RIPA buffer and the lysates were separated by SDS-PAGE and subjected to a western blot analysis with anti-ERK5 antibody (N-19).





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