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Fig. 1. Expression of genes encoding HIF-1{alpha} (HIF1A) and HIF-2{alpha} (EPAS1) in Z-33, REH and Hep3B cells. The human B-cell acute lymphoblastic leukemia cell lines Z-33 and REH as well as the hepatoblastoma cell line Hep3B (Hep) were cultured in normoxia. Ribonuclease protection analysis using 30 µg of total RNA revealed no expression of HIF1A RNA in Z-33 in contrast to REH and Hep3B. The latter depicted strong signal intensity for EPAS1 whereas very little EPAS1 signal was obtained in Z-33 or REH cells. Probing against U6 small nuclear RNA (RNU6) was used for loading control. Complete ribonuclease digestion of 32P-riboprobes in the absence of added RNA was demonstrated on the right lane (Con).





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