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Fig. 2. Agarose gel electrophoresis of PCR products indicating homozygous deletion of the HIF-1{alpha} gene in Z-33. (A) Genomic PCR of DNA of Z-33 and REH was performed for the HIF-1{alpha} gene (HIF1A exon/intron-3, lane 3; HIF1A exon-12, lane 4) and HIF-1{alpha} flanking gene regions: proximal (prox) to the HIF-1{alpha} locus marker D14S592, lane 1 and D14S1258, lane 2; distal (dist) D14S1334, lane 5 and D14S183, lane 6; ß-globin, lane 7; and 100-bp DNA size marker, lane M. Fig. 2B shows the positions of the different markers relative to the HIF-1{alpha} gene on the chromosome band 14 q23.1 and q23.2, numbered according to the lanes.





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