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Fig. 3. Hypoxia induces RBM3 and CIRP in HIF-1
-deficient Z-33 cells as well as in murine ARNT-deficient Hepa-1 c4 cells. Z-33, REH, Hepa-1 wt and c4 cells were cultured in parallel in normoxia or 1% oxygen for 24 hours. (A,B) Whole-cell lysates were isolated and subjected to western blot analysis for RBM3, CIRP and HIF-1 in Z-33 and REH cells. To control sample loading and transfer, the blots were stripped and reprobed for ß-actin. (C) RNA was isolated and subjected to real-time RT-PCR analysis. RBM3 (R), CIRP (C), and VEGF (V) RNA levels were normalized to RPL13a RNA levels and are expressed as fold change in the different experiment samples compared with the corresponding normoxic samples (mean±s.e.m., n=3).
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