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Fig. 5. Mild hypoxia as well as hypothermia increase RBM3 and CIRP protein but not HIF-1{alpha}, whereas desferrioxamine (DFO) does not affect RBM3 and CIRP protein. HeLa cells were cultured in parallel in normoxia (N), 8% oxygen (8%), DFO or 32°C-hypothermia (cold) for 24 hours. Whole-cell lysates were isolated and subjected to western blot analysis for RBM3, CIRP and HIF-1{alpha}. To control sample loading and transfer, the blots were stripped and reprobed for ß-actin.





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