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Fig. 3. Development of IFM fibres in hdp³ flies. (A) Fusion of myoblasts (red, Dmef2 immuno-localised expression) with the DLM templates (green, Act88F-GFP) in the hdp³ mutant. The fusion process is not affected (yellow colour is as a result of overlaying of the red and green). Anterior to the lower left-hand corner, posterior to upper right-hand corner. Bar, 0.076 mm. (B) Wild-type DLM myotubes at around 40 hours APF (immunolocalised with Dmef2 expression). Fibres are elongated with syncytial nuclei arrayed in lines. Bar, 0.09 mm. (C) hdp³ myotubes at the same stage with the nuclei in arrays, indicating that myoblast fusion is not affected. hdp³ fibres are shorter than wildtype and never extend completely to reach the attachment sites. Bar, 0.115 mm. For both B and C, anterior is to the top; dorsal towards the left. (D) Wild-type IFM development at 42 hours APF monitored with Act88F-GFP expression. The fibres completely extend to the cuticle. The star indicates a DLM and `I' overlays one of the DVM. (E) hdp³ mutant DLM and DVM (I) fibres at 42 hours APF are shorter than wildtype and start degrading (arrow) from the fibre ends. (F) hdp³ DLM fibres at 46-48 hours APF. Fibres are clumped together and pulled to sides (arrowheads) and completely degrade with time. No fibres are seen in newly eclosed adults (see Fig. 7A). Anterior to the right-hand side for panels D-F. Only one hemi-segment shown in all figures. Bars, 0.1 mm (D-F).

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