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Fig. 4. Myofibril assembly in wild-type and hdp³ flies. Immunolocalisation of myosin (green: FITC-labelled antibody) and F-actin filaments (red, rhodamine-phalloidin labelled). (A) Wild-type myofibrils: single myofibrils are visible with thick (arrowheads showing two alternate A-band regions) and thin (arrows indicating two alternate I-band regions) filaments assembled as clearly demarcated repetitive I-A-I bands. (B) hdp³ mutant myofibrils are clumped as a bundle of barely distinguishable myofibrils with no clear sarcomere-like structures. Thick and thin filaments are seen, but scattered without a definite structure. The asterisk indicates the degenerating region of the developing fibre. Bars, 1.6 µm.

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