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Fig. 4. Nuclear structures induced by {Delta}Ext/R4Tth are found in cells at various cell cycle stages. (A,B) COS-1 cells transfected with {Delta}Ext/R4Tth were incubated with BrdU for 18 hours, then fixed and labeled with anti-BrdU (red) and anti-FGFR4 (green) antibodies. Yellow color indicates overlap between red and green signal. The {Delta}Ext/R4Tth-transfected cell has not incorporated BrdU in A, whereas the transfected cell in B has. (C) Nuclear spots could be observed in pair of cells, indicating that they arose by division of a single transfected cell. Scale bar, 10 µm.





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