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Fig. 5. {Delta}Ext/R4Tth forms spots along the NE that stain for protein disulfide isomerase. COS-1 cells transfected with {Delta}Ext/R4Tth were fixed, labeled with antibodies and examined by confocal microscopy. (A) Three sections of a cell labeled with anti-FGFR4 antibodies and FITC-conjugated secondary antibodies. (Low) Image taken close to the coverslip. (Middle) Image taken through the middle of the nucleus. (Top) Image taken at the top of the cell/nucleus. (B) Double labeling with anti-FGFR4 (green) and anti-nucleoporin p62 (red) antibodies. (C) Double labeling with anti-FGFR4 (green) and anti-PDI (red) antibodies. Yellow color indicates the overlap of red and green signal. Scale bar, 10 µm.





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