|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. Lectin binding by matrigel cultures. Matrigel cultures, initiated as described in the legend to Fig. 2 above, were incubated for 20 hours in Phenol Red-free medium supplemented with a lectin (as specified in A, B, C or D below), in addition to insulin, transferrin, hydrocortisone and Lucifer Yellow (as described in Fig. 3). Confocal images of matrigel cultures maintained 20 hours with particular lectins. (A) TRITC T. vulgare (30 µg/ml) (shown as red) + Lucifer Yellow (green) (insert shows a culture maintained for 20 hours as above with additional brefeldin A, 30 µg/ml), (B) FITC-Lotus Tetragonolobus purpurea (30 µg/ml) (green), (C) FITC-Dolichos biflorous (50 µg/ml) (green), and (D) TRITC-glycine max (30 µg/ml) (red) + Lucifer Yellow (green) are illustrated, as overlayed on bright-field images. The lumenal space shown in A is apparently still developing, not being contiguous, as indicated by the TRITC T. vulgare staining of the apical membrane, which sections off a portion of the lumen from the rest of the lumenal space. Bar in A, 50 µm (same scale for the insert in A).
|
