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Fig. 8. Cyst formation by matrigel cultures. Matrigel cultures incubated with 8-Br-cAMP were examined (a) under the inverted microscope, (b) after the preparation of paraffin sections, as well as (c) under the confocal microscope following incubation with Lucifer Yellow. Bars, 50 µm (a-c). The effects of 8-Br-cAMP and TPA on (d) cyst formation and (e) tubule formation were quantitated as described in Materials and Methods. An effect was found to be significant (*) when P<0.05. (f) Effect of 8-Br-cAMP on the level of the mRNA for the Na,K-ATPase
subunit, the Na,K-ATPase ß subunit, mitochondrial PEPCK, and ß-actin was determined by northern analysis.