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Fig. 1. bgs4⁺ cwg1⁺and orb11⁺ are the same gene. (A) bgs4⁺ suppresses the lethal thermosensitive phenotype of cwg1-1 mutant cells. Wild-type (leu1-32 h^–) and cwg1-1 (cwg1-1 leu1-32 h^–) cells transformed with the empty plasmid pAL-KS⁺ or the plasmid pAL-bgs4⁺ (pJG1) and grown at 28°C were streaked out on EMM plates and incubated at 37°C for 4 days. (B) bgs4⁺ suppresses the in vitro GS defect of cell extracts from cwg1-1 cells grown at 37°C. The same cells as in A were grown on EMM + 1.2 M sorbitol liquid medium at 37°C and the GS activity of cell extracts was analyzed at 30°C. Values in parentheses are the specific activity average, calculated from three independent extracts. (C) Both homozygous cwg1-1/cwg1-1 and heterozygous cwg1-1/bgs4 diploids present the same thermosensitive spherical morphology. Phase-contrast and Calcofluor white (CW)-stained UV micrographs of log-phase cells grown on EMM liquid medium at 37°C for 12 hours. (D) bgs4⁺ suppresses the thermosensitive morphology of orb11-59 cells. Wild-type and orb11-59 cells transformed with the indicated plasmids were grown at 37°C for 24 hours and visualized as in C. Bar, 10 µm.

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