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Fig. 4. CAP350 redirects PPAR ${alpha}$ subcellular localization. (A) NIH3T3 cells were transfected separately with expression plasmids for PPAR ${alpha}$ (panels A-C) or CAP350 (panels D-F) and subjected to immunostaining with anti-PPAR ${alpha}$ antibody (green) or anti-c-Myc antibody (red) to detect tagged CAP350, as indicated. Nuclei were stained with Hoechst. Panels C and F show the respective merged images. Representative images are shown. PPAR ${alpha}$ is found distributed throughout the nucleus and is absent from the cytoplasm. CAP350 is present in the nucleus and concentrates in discrete subnuclear foci, as well as in the perinuclear region and on cytoskeletal microfilaments. (See also movie 1 in supplementary material.) (B) NIH3T3 cells were cotransfected with expression vectors for PPAR ${alpha}$ and CAP350, which were visualized in the same cell. Panel D is the merged image in which colocalization of PPAR ${alpha}$ and CAP350 is shown in yellow. (See also Movie 2 in supplementary material.) Bars, 10 µm.

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