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Fig. 5. CAP350 does not affect RXR ${alpha}$ or TR ${alpha}$ subcellular localization. Representative images of NIH3T3 cells transfected with an expression vector for RXR ${alpha}$ alone (A-C) or TR ${alpha}$ alone (J-L) or cotransfected with expression vectors for RXR ${alpha}$ and CAP350 (D-F) or TR ${alpha}$ and CAP350 (M-O), and analyzed by indirect immunofluoresence microscopy with anti-RXR ${alpha}$ antibody or anti-TR ${alpha}$ antibody (green) and anti-c-Myc antibody (red) to detect tagged CAP350. The merged images indicate that CAP350 does not alter the subcellular localization of RXR ${alpha}$ or TR ${alpha}$ (F and O, respectively). Nuclei were detected by staining with Hoechst (B and K). (G-I) PPAR ${alpha}$ ligand does not affect the subcellular relocalization of PPAR ${alpha}$ in the presence of CAP350. NIH3T3 cells were transfected with expression vectors for PPAR ${alpha}$ and CAP350 in the presence of the PPAR ${alpha}$ ligand Panagonist B and subjected to immunostaining with anti-PPAR ${alpha}$ antibody (green) and anti-c-Myc antibody (red) to detect tagged CAP350. The merged image (I) shows that ligand does not alter the colocalization of PPAR ${alpha}$ and CAP350. Bars, 10 µm.

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