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Fig. 1. Hob1 interacts with Nak1. Epitope-tagged proteins were expressed in wild-type (RL143) cells using nmt1 promoter expression plasmids, and were assayed for expression by western-blot analysis using anti-Myc (9E10) or anti-HA (12CA5) monoclonal antibodies (top). Extracts were immunoprecipitated with anti-HA antibody and equal portions of the immunoprecipitates were probed with anti-HA antibody and anti-Myc antibody (bottom). (A) Control vectors (lane 1), Myc-Nak1 alone (lane 2), HA-Hob1 and Myc-Nak1 (lane 3), HA-Hob1 alone (lane 4), and HA-Hob1 and Myc-Nak^1-562 (lacking the CTR; lane 5). (B) Control vectors (lane 1), HA-Nak1 (lane 2), HA-Nak1 and Myc-Hob1 (lane 3), HA-Nak1 and Myc-Hob1^1-281 (lane 4), Myc-Hob1 (lane 5), and Myc-Hob1^1-281 alone (lane 6).

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