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Fig. 2. Nak1 and Hob1 interact with Wsp1. Extracts (top) and anti-HA immunoprecipitates (bottom) from wild-type (RL143) cells expressing HA- or Myc-tagged proteins from nmt1 promoter expression plasmids were assayed by western-blot analysis using anti-Myc (9E10) or anti-HA (12CA5) monoclonal antibodies. (A) Control vectors (lane 1), Wsp1-HA alone (lane 2), Wsp1-HA and Myc-Hob1 (lane 3), and Myc-Hob1 alone (lane 4). (B) Control vectors (lane 1), Myc-Wsp1 (lane 2), HA-Hob1 and MycWsp1 (lane 3), HA-Hob1^1-281 and Myc-Wsp1 (lane 4), and HA-Hob1^275-466 and Myc-Wsp1 (lane5). (C) Control vectors (lane 1), Myc-Wsp1 alone (lane 2) and Myc-Wsp1 and HA-Nak1 (lane 3).

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