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Fig. 6. Hob1 overexpression in wild-type and nak1-repressed cells. (A) Wild-type RL143 cells containing a control plasmid or expressing HA-Hob1, HA-Hob1^1-281 or HA-Hob1^275-466 from adh1 promoter expression plasmids were grown on selective medium at 30°C for 3 days. (B) TYH1 (nmt1-nak1) cells containing a control plasmid or expressing HA-Nak1, HA-Hob1, HA-Hob1^1-281 or HA-Hob1^275-466 were grown on minimal medium plus 100 µg ml^–1 thiamine for 2 days at 30°C and examined by differential interference contrast microscopy. (C) TYH1 (nmt1-nak1) cells expressing the indicated proteins were grown on minimal medium plates containing 100 µg ml^–1 thiamine for 5 days at 30°C.

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